In one in vitro study MCF-7 tumor colony formation rate was reduced by 60% 

in CCE- and CME-treated cells and nearly completely inhibited (99%) 

by FVE treatment，regardless of the hormone receptor status of the cancer cells.. 【2】

According to Prof. Xu Fu of the horticulture department of Taiwan University, 

after heating the mushroom for 15 minutes under 121℃  and also heating 

30 minutes under 95℃, the anticancer substance of FIP-fve still remain intact.  

That protein had not been destroyed. In further investigation, after high heat 

treatment, the effect of FIP-fve on the spleen cells and the the production of 

the interferin, IFN-r of the lab mice, that the anticancer ingredient FIP-fve 

of the mushroom possesses the quality of high temperature endurance.

在一體外試驗， 三種菇類：CME，雞腿菇，金針菇之提取物對人類乳腺癌，無論

是正性雌二醇受體，或是負性雌二醇受體都有效。尤其是金針菇的提取物能夠

抑制99%的人類乳腺癌細胞。

台大園藝系許輔教授的研究中顯示在121℃ 15分鐘及95℃ 30分鐘處理後，

其抗癌物質FIP-fve仍維持原有之分子量，且明確可辨該蛋白質未遭分解。

再進一步測定高溫處理後FIP-fve對小鼠脾細胞增生效應以及小鼠脾細胞

產生干擾素（Interferin, IFN-r）的影響，發現FIP-fve有很高的耐熱性，